Optimization of the Culture Medium Composition to Improve the Production of Hyoscyamine in Elicited Datura stramonium L. Hairy Roots Using the Response Surface Methodology (RSM)

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Optimization of the Culture Medium Composition to Improve the Production of Hyoscyamine in Elicited Datura stramonium L. Hairy Roots Using the Response Surface Methodology (RSM)

Traditionally, optimization in biological analyses has been carried out by monitoring the influence of one factor at a time; this technique is called one-variable-at-a-time. The disadvantage of this technique is that it does not include any interactive effects among the variables studied and requires a large number of experiments. Therefore, in recent years, the Response Surface Methodology (RS...

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Background: The use of the desirability function approach combined with the response surface methodology (RSM), also called Desirability Optimization Methodology (DOM), has been successfully applied to solve medical, chemical, and technological questions. It is particularly effi cient for the determination of the optimal conditions in natural or industrial processes involving d...

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Effect of Culture Medium on Hyoscyamine Production from Four Datura Sp Hairy Roots

Tropane alkaloids present a great economic value in particular for pharmaceutical industry. The culture of Datura sp hairy roots obtained by inoculation with A4 Agrobacterium rhizogenes offers promising prospects for alkaloids in-vitro production. The objective of our study is to try optimizing hyoscyamine production exploiting the composition of mineral fraction of the culture media. On the wh...

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ژورنال

عنوان ژورنال: International Journal of Molecular Sciences

سال: 2010

ISSN: 1422-0067

DOI: 10.3390/ijms11114726